Actualizar DataTable a la base de datos MySql C#

Necesitas configurar SelectCommand , DeleteCommand , UpdateCommand y InsertCommand propiedades de DataAdapter. Puede usar MySqlCommandBuilder para completar tres objetos de comando (Seleccionar, Eliminar, Actualizar).

MySqlConnection connection = new MySqlConnection(MyConString);
string sQuery="Select * from Table";

MySqlDataAdapter myDA = new MySqlDataAdapter(sQuery, connection);
MySqlCommandBuilder cmb = new MySqlCommandBuilder(myDA);

DataTable MyDT = new DataTable()// <- My DataTable
myDA.Fill(MyDT);

//Add new rows or delete/update existing one
//and update the DataTable using 

myDA.Update(MyDT);